Journal: iScience

Article Title: MiR-25 blunts autophagy and promotes the survival of Mycobacterium tuberculosis by regulating NPC1

doi: 10.1016/j.isci.2022.104279

Figure Lengend Snippet: The miR-25 level is induced by Bacillus Calmette–Guérin and Mtb infection (A and B) Quantitative real-time PCR (qRT-PCR) detection of the expression of miRNAs in the lungs of the control group or mice (n = 3) infected with 4.4 × 10 5 CFU BCG via tail vein for 3 days (A) and 7 days (B). (C) qRT-PCR detection of the expression of miRNAs in THP-1 cells of the control group or cells infected with BCG or H37Rv. (D) qRT-PCR detection of the expression of miR-25 in THP-1 cells infected with BCG at the indicated MOIs for 24 h. (E) qRT-PCR detection of the expression of miR-25 in THP-1 cells infected with BCG for 6, 12 and 24 h. (F) qRT-PCR detection of the expression of miR-25 in THP-1 cells infected with H37Rv at the indicated MOIs for 24 h. (G) qRT-PCR detection of the expression of miR-25 in THP-1 cells infected with H37Rv for 6, 12 and 24 h. Data are presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ns, not significant.

Article Snippet: The tailing method for rapid batch detection of miRNA expression, reverse transcription of miRNA using miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Tiangen, KR21), quantitative analysis of miRNA using miRcute Plus miRNA qPCR Detection Kit (SYBR Green) (Tiangen, FP411). (miR-146a-F:5′-TTGAGAACTGAATTCCATGGGTT-3′,miR-20a-F:5′-TAAAGTGCTTATAGTGCAGGTAG-3′,miR-25-F:5′-CATTGCACTTGTCTCGGTCTGA-3′,miR-186-F:5′-CAAAGAATTCTCCTTTTGGGCT-3′,miR-199a-F:5′-CCCAGTATTCAGACTACCTGTTC-3′,miR-199b-F:5′-CCCAGTGTTTAGACTACCTGTTC-3′,miR-320b-F:5′-AAAAGCTGGGTTGAGAGGGCGA-3′,miR-429-F:5′-GTAATACTGTCTGGTAAAACCGT-3′).

Techniques: Infection, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Control

Journal: iScience

Article Title: MiR-25 blunts autophagy and promotes the survival of Mycobacterium tuberculosis by regulating NPC1

doi: 10.1016/j.isci.2022.104279

Figure Lengend Snippet:

Article Snippet: The tailing method for rapid batch detection of miRNA expression, reverse transcription of miRNA using miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Tiangen, KR21), quantitative analysis of miRNA using miRcute Plus miRNA qPCR Detection Kit (SYBR Green) (Tiangen, FP411). (miR-146a-F:5′-TTGAGAACTGAATTCCATGGGTT-3′,miR-20a-F:5′-TAAAGTGCTTATAGTGCAGGTAG-3′,miR-25-F:5′-CATTGCACTTGTCTCGGTCTGA-3′,miR-186-F:5′-CAAAGAATTCTCCTTTTGGGCT-3′,miR-199a-F:5′-CCCAGTATTCAGACTACCTGTTC-3′,miR-199b-F:5′-CCCAGTGTTTAGACTACCTGTTC-3′,miR-320b-F:5′-AAAAGCTGGGTTGAGAGGGCGA-3′,miR-429-F:5′-GTAATACTGTCTGGTAAAACCGT-3′).

Techniques: Virus, Recombinant, Transfection, Lysis, cDNA Synthesis, SYBR Green Assay, Control, Software